The technique based on stable isotope labeling and mass spectrometry is one of the main supporting techniques for protein quantification. 目前,应用稳定同位素标记技术定量蛋白质表达水平是最准确的方法之一,且联合色谱质谱技术更具优势。
In this paper, these novel protein isotope labeling methods and alternative protein expression systems are reviewed. 本文旨在对这些蛋白质标记表达新技术的方法及应用予以逐一介绍。
Recently, some new isotope protein labeling strategies and new protein expression systems have emerged as tools for studying large proteins by sol. 至今已发展的同位素标记技术和蛋白质表达系统已获得了广泛的应用,对蛋白质的核磁共振研究起到了巨大的推动作用。
Development of Protein Quantitative Algorithms Based on Stable Isotope Labeling and Mass Spectrometric Analysis 基于稳定同位素标记与质谱分析的蛋白质定量算法研究进展
Protein isotope labeling is crucial for NMR studies of the three-dimensional structure and dynamics of proteins in solution. 在运用核磁共振技术研究蛋白质溶液三维结构和动态特性中,蛋白质的同位素标记表达是研究的关键。
RT PCR with blotting and isotope labeling were used to detect minor amount of mRNA. 应用RT-PCR技术联合印迹杂交和同位素掺入法对微量mRNA检测。
The angiotensinogen mRNA in leukocytes can be detected by RT PCR with blotting and isotope labeling. 将RT-PCR技术同印迹杂交或同位素掺入法联合应用能提高血管紧张素原微量mRNA检测灵敏度。
RT PCR with blotting or isotope labeling was an effective method for quantitative analysis of minor amount of mRNA. RT-PCR联合印迹杂交或同位素掺入法是微量mRNA定量分析的有效方法。
Quantitative Proteomics Using Isotope Labeling Techniques and Its Recent Application 定量蛋白质组同位素标记技术及应用
The ability to quantify differences in protein expression and post-translational modifications using stable isotope labeling has been achieved. 在此基础上,实现了对表达的蛋白质差异和翻译后修饰的蛋白质差异进行精确定量分析。
The stable isotope labeling and the isotope coded affinity tags ( ICAT) strategy provided an ideal method for accurately quantitating the low copy number proteins which play a key role in cells or tissues. 从稳定同位素标记到ICAT战略的提出,为准确定量在细胞或组织中发挥重要调节功能的低丰度蛋白质提供了一个较为理想的方法。
In the review, the author stated the application of stable isotopic labeling in quantitative proteomics in the following aspects, such as stable isotope dilution technique and stable isotope chemical labeling. 本文对稳定同位素化学标记结合质谱技术在定量蛋白质组学中的研究进展进行了评述。
More efforts have been focused on quantitative proteomic techniques recently. The stable isotope labeling technique provided an ideal method for accurately quantitating the low abundance proteins which play a key role in cells or tissues. 近几年来,定量蛋白质组的技术发展很快,稳定同位素标记技术的提出,为准确定量在细胞或组织体系中发挥重要功能的低丰度蛋白质提供了一个理想的方法。
The concentration-response relationships were marked and consistent with the results obtained by the isotope labeling precursor incorporation assay. 本法测得的抗癌活性量-效关系十分显著,与同位素核酸前体参入法结果吻合。
A Strategy for the Establishment of Plasma Proteome Database Based on Stable Isotope Labeling and FT-MS 基于稳定同位素标记与FT-MS技术的血浆蛋白质组数据集
The most common techniques in this field include the comparison of the intensities based on gel staining spots and the integration of mass spectrometric ( MS) peak intensities with stable isotope labeling. 该领域最常用的技术是比较染色后的二维凝胶上蛋白点的光密度值或综合同位素标记后的质谱峰强度方法。
Proteome analysis includes two dimensional electrophoresis, two dimensional liquid chromatography, surface enhanced affinity capture and isotope mass labeling based techniques followed by mass spectrum identification. 蛋白质组学技术包括二维电泳、二维液相层析、表面增强亲和捕获和稳定同位素质量标签技术等与质谱的联用。
Advance in isotope labeling techniques and recent biological applications are reviewed, further de-velopment based on quantitative proteomics is discussed. 对近年来定量蛋白质组学同位素标记技术和应用研究所取得的重要进展以及最新的发展动态进行了综述。
Application of Quantitative Proteomics Expression Analysis Using Stable Isotope Labeling 稳定同位素标记方法在蛋白质组学定量分析中的应用
Identification of Agmatine and Its Acetylation Metabolites in Rat Urine by Stable Isotope Labeling Coupled Ion Trap Mass Spectrometry 稳定同位素标记结合离子阱质谱鉴别大鼠尿中胍丁胺及其乙酰化代谢产物
To investigate the catalytic mechanism carefully by isotope labeling experiment, we found out that the oxygen atom in the epoxide was all from molecular oxygen but not water. 本论文通过同位素实验对催化反应机理进行了细致的研究,发现环氧化物中的氧全部来源于氧气,而不是来源于水。
Colloidal gold technique is a novel labeling technique after isotope, fluorescin and enzyme labeling, which has the merits such as easy, rapid, sensitive and ready-to-read. 胶体金技术是继同位素、荧光素、酶标记技术之后的一种新型标记技术,它具有快速、敏感、便捷、不需特殊设备、结果判断直观等优点。